| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
A complementary method for production of tetraploid Crassostrea gigas using crosses between diploids and tetraploids with cytochalasin B treatments
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| Autores: |
Mccombie, Helen
Ledu, Christophe
Phelipot, Pascal
Lapegue, Sylvie
Boudry, Pierre
Gerard, Andre
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| Data: |
2005-08
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| Ano: |
2005
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| Palavras-chave: |
Oyster breeding
Gynogenesis
Polar body
Cytochalasin B
Polyploidy
Crassostrea gigas
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| Resumo: |
We present a new method to produce tetraploid Crassostrea gigas by cytochalasin B inhibition of polar body 2 expulsion in diploid females crossed with tetraploid males. This offers a means of direct introgression of genetic characters from selected diploid to tetraploid lines, avoiding a triploid step. Offspring larval ploidy shifted over time and depended on size, with tetraploids more frequent among the smaller larvae and triploids among the large. Viable tetraploids were found at 4 and 6 months, indicating the technique was successful. The possibility that gynogenesis occurred was tested by microsatellite analysis to confirm the presence of paternally inherited alleles. These were present in all animals of the 2n x 4n + CB (female first) cross. However, a 4n x 2n + CB cross produced triploids, including some gynogens. Our method illustrates for the first time that diploid C. gigas eggs, if selected for large size, can give viable tetraploid offspring.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/2005/publication-632.pdf
DOI:10.1007/s10126-004-0440-2
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| Editor: |
Springer
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| Relação: |
http://archimer.ifremer.fr/doc/00000/632/
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| Formato: |
application/pdf
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| Fonte: |
Marine Biotechnology (1436-2228) (Springer), 2005-08 , Vol. 7 , N. 4 , P. 318-330
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| Direitos: |
2005 Publisher: Springer-Verlag New York
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